Wide-field Hyperspectral imaging

Another area I contributed to is wide-field Hyperspectral imaging. Hyperspectral imaging has a number of applications in bio-imaging. Many biological molecules have distinct spectral signatures that may be sensitive to local biochemical microenvironments. Hyperspectral imaging can also be used to resolve spectrally overlapping fluorescent labels enabling highly multiplexed fluorescence imaging. Moreover, many non-fluorescent analytes utilizing their complex vibronic Raman spectra may also be realized using hyperspectral imaging. To this end, I have worked on developing two wide-field hyperspectral imaging systems. The first system was built to demonstrate wide-field hyperspectral imaging in 3D [a]. This was achieved by combining structured light illumination with a Sagnac interferometer. Structured light modulated depth information while the interferometer modulated the spectral information. Depth-resolved hyperspectral images were then computationally demodulated. The second system was designed to utilize compressive sensing for wide-field interferometric (or Fourier transform) imaging spectroscopy [b]. To this end, we designed a novel common-path imaging interferometer with a uniform phase profile over the field of view. The common-path architecture made interferometric measurements stable, while the uniform phase profile enabled strategic spectral sampling for compressive measurements. The proposed design outperforms state-of-the-art Sagnac interferometric spectrometers by an order to two orders of magnitudes.

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