High-throughput Raman imaging

Raman imaging can be used to extract chemical information of cell and tissue specimen, label-free. Label-free Raman based non-destructive pathology approaches have been proposed with the bottleneck being the imaging time. Imaging throughput can be improved by substituting point-scanning spectral imagers with wide-field geometries. However, wide-field techniques require engineering power efficient excitation technologies to maintain the excitation power per point. To this end, I designed two wide-field Raman systems. The first system is based on light-sheet illumination. Light-sheet illumination utilizes laser power since the direction of propagation is along the field of view (FOV). The entire FOV’s spectral signatures were captured using the new common-path interferometer. The second implementation is based on waveguide total-internal-reflection(TIR) illumination. Waveguides are perfect platforms to illuminate large FOVs using the evanescent field. In collaboration with professor Ahluwalia’s group at the University of Tromso in Norway, we developed a waveguide-based, wide-field Raman microscope.

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